Scientific & Technical Consulting for Early-Stage Biotech

Diluks LLC

Helping teams move from therapeutic concept to CRO-ready DNA designs, executable study plans, and decision-grade preclinical data.

Senior-level technical support before a company commits substantial money to DNA synthesis, vector production, CRO studies, or diligence decisions.

Overview

Built for small, virtual, and venture-backed biotech teams.

Focused support for early-stage teams that need senior technical judgment without immediately hiring full-time platform or preclinical leadership.

Who we help

Early-stage biotech, gene therapy, genetic medicine, platform biology, and preclinical teams.

What we do

DNA design, promoter development, vector strategy, study design, CRO selection and management, and technical diligence.

Typical deliverables

Construct maps, study plans, CRO quote comparisons, data review memos, SOPs, and decision-gate recommendations.

Relevant expertise

Gene therapy; AAV, lentiviral, and CAR-T vector platforms; promoter/enhancer and regulatory element design; in vivo pharmacology; assay development; lab and project team leadership; translational strategy.

Engagement models

Hourly advisory, fixed-scope work package, capped hourly project, monthly retainer, or diligence sprint.

Credentials

Relevant Experience & Credentials

Industry and academic experience across molecular biology, viral vectors, preclinical development, CRO/CDMO execution, and portfolio decision support.

20+ years across molecular biology, virology, immunology, oncology, and neuroscience.

10+ years leading development of AAV platform for CNS gene therapy and lentiviral CAR-T platform at Novartis.

Project team leadership from target discovery through preclinical development.

Lab head with a record of building teams, platforms, and research capabilities from scratch.

Consulting support for startup biotech companies funded by major VC groups with focus on vector design, assay development, and study planning.

CRO/CDMO management, governance decision support, technical diligence, portfolio strategy, and IP generation.

Capabilities

Core capabilities and outputs

Practical deliverables for teams preparing construct designs, CRO-facing studies, diligence reviews, or ongoing preclinical execution.

1

DNA construct design

For companies needing plasmids, AAV/LVV constructs, promoter/enhancer designs, reporters, barcodes, or expression cassettes.

  • Construct architecture recommendation
  • Annotated DNA maps
  • Sequence design review
  • Codon/regulatory element review
  • Cloning/vendor-ready design package
  • Risk assessment: repeats, recombination, cryptic splicing, CpG, payload limits, LTR and ITR
2

CRO-ready study design

For teams preparing in vitro, in vivo, biodistribution, expression, potency, or exploratory pharmacology studies.

  • Study objective and decision gate
  • Experimental design
  • Arms, controls, endpoints, and sample size logic
  • Materials list
  • Data package expectations
3

CRO selection and management

For small teams that know what they need but cannot manage execution.

  • CRO shortlist
  • Quote comparison
  • Technical evaluation
  • Kickoff meeting
  • Weekly oversight
  • Data review, deviation management, and final report review
4

Gene therapy technical diligence

For investors, company founders, or BD teams evaluating an asset.

  • Vector/design review
  • Preclinical package analysis
  • Manufacturability risks
  • Translational risks
  • Competitive landscape
  • Key risks and recommended next experiments
5

Fractional scientific lead

For seed-stage companies without a full-time head of platform/preclinical.

  • Standing weekly advisory
  • Study planning
  • Vendor oversight
  • Technical slide review
  • Founder/investor support
  • Internal program strategy
6

AI / Data / Bioinformatics

Additional capability for evidence generation, prioritization, and data-analysis workflows.

  • Promoter/enhancer discovery and prioritization
  • Construct risk screen
  • Literature and competitive landscape synthesis
  • Target expression evidence package
  • Cross-species conservation review
  • Single-cell/ATAC/RNA-seq workflow design and analysis support
Value proposition

De-risk execution before major experimental spend.

Support for teams that need stronger construct logic, cleaner study design, better CRO scoping, or diligence-grade technical assessment before committing budget and timelines.

Contact

Dmitriy Lukashev, PhD

Diluks LLC - Scientific & Technical Consulting for Early-Stage Biotech

Contact information

Engagement types

Available for hourly advisory, fixed-scope work packages, capped hourly projects, monthly retainers, diligence sprints, CRO-facing workstreams, and fractional scientific leadership.